Flow Cytometry Wavelength Chart
Uvod:
Flow cytometry is a powerful technique used to analyze and characterize cells and particles. It involves the use of laser light of different wavelengths to excite fluorochromes attached to the cells, and then measure their emission signals. A key parameter in flow cytometry is the selection of appropriate lasers and fluorochromes, which requires knowledge of the excitation and emission spectra of each fluorochrome. In this article, we present a wavelength chart for commonly used fluorochromes in flow cytometry.
Wavelength Chart:
1. Ultraviolet (UV) Region:
– Common laser source: UV laser (e.g., 355 nm)
– Fluorochromes: Pacific Blue, DAPI, Hoechst 33342
– Excitation range: around 350-365 nm
– Emission range: around 450-470 nm
2. Blue Region:
– Common laser source: Blue laser (e.g., 488 nm)
– Fluorochromes: FITC, PE, PerCP, PerCP-Cy5.5, PE-Cy5, PE-Cy7, APC
– Excitation range: around 488-505 nm
– Emission range: around 515-780 nm
3. Green-Yellow Region:
– Common laser source: Yellow-green laser (e.g., 561 nm)
– Fluorochromes: PE-Cy5.5, PE-Cy7, PE-Texas Red, PE-TR, PE-Alexa Fluor 700
– Excitation range: around 551-575 nm
– Emission range: around 600-780 nm
4. Red Region:
– Common laser source: Red laser (e.g., 633 nm)
– Fluorochromes: APC, APC-Cy7, APC-H7, APC-eFluor 780, Alexa Fluor 647
– Excitation range: around 633-650 nm
– Emission range: around 660-780 nm
5. Infrared Region:
– Common laser source: Infrared laser (e.g., 785 nm)
– Fluorochromes: APC-eFluor 780, APC-H7, Cy5.5, Cy7, Pacific Orange, Alexa Fluor 700, Alexa Fluor 750
– Excitation range: around 750-785 nm
– Emission range: around 800-850 nm
Zaključek:
The wavelength chart provided in this article serves as a guide for selecting appropriate lasers and fluorochromes in flow cytometry experiments. It summarizes the excitation and emission ranges of commonly used fluorochromes, helping researchers make informed decisions when designing their experiments. By understanding the properties of different fluorochromes and matching them with the appropriate laser sources, flow cytometry users can optimize their experimental setups and obtain accurate and reliable data.
